Clik here to view.
Science Hack Day SF 2015 - Amino Acid Dependence of alpha Helix Length
Last year at Science Hack Day SF I led and ardent band of marauders into battle in an attempt to create a simple method to sequence DNA. Though we accomplished some stuff it was alot of work! This year...
View ArticleI transplanted someone else's microbiome in(on)to my body and it was so...
This is the story of a Speculative Science experiment. The data and results are meant to be a guide and not meant to withstand rigorous peer review (though I did my best to be as rigorous as...
View ArticleI transplanted someone else's microbiome in(on)to my body and it was so...
You can follow along Here for my daily questionaire and Here there is a diary near the bottomTuesday Feb 16th - Antibiotics Day One, Experiment Day One The first day I would be taking antibiotics....
View ArticleClik here to view.
I transplanted someone else's microbiome in(on)to my body and it was so...
This is a case study of a 35 year old caucasian Male of European ancestry living in the United States, a maternal Haplogroup of H1e1a and a paternal haplogroup of I1*. NOD2 Genotype SNP(rs2066844) CC...
View ArticleClik here to view.
Green Flourescent Protein(GFP) Beer, a short story
Yeast is used in alot of food and drink. It is used to make some of my favorite drinks, beer and whiskey. I started engineering Yeast about 1.5 years ago. I thought it would be a useful skill to have...
View ArticleClik here to view.
Let's start at the beginning
I want the world to be like I see it in my head. In April 2015 I was sitting around thinking. I like to sit around and think. And I began to wonder how genetic engineering technologies would be used in...
View ArticleClik here to view.
How to Genetically Engineer a Human in Your Garage - Part I
My name is Josiah Paul Zayner. If my name sounds "religious" that's because it is, Josiah was a King of Judah in the Hebrew.Christian Bible, Paul was an apostle of Jesus. I have three brothers...
View ArticleClik here to view.
How to Genetically Engineer a Human in Your Garage - Part II - DNA got what I...
Ohhh babbbyyy, you, you got what I need To embark on an experiment to start genetically modifying myself I needed to obtain DNA and not just any DNA, some that was specifically encoded to work with...
View ArticleClik here to view.
How to Genetically Engineer a Human in Your Garage - Part III - The first...
Whenever I start to work on something I have trouble sleeping. The problem is that I am always working on something so I always have trouble sleeping. It's not worry or anxiety, it's hard to describe...
View ArticleDIY Guide to Creating a Peptide Immunotherapy Cancer Vaccine
I received an email over a year ago from Lars. His wife Dyanne, a never smoker, had developed Non-Small Cell Lung Cancer. He was reaching out to me to see if CRISPR would be a viable way to try and...
View ArticleClik here to view.
The First Attempt At Human CRISPR Gene Editing
The first attempt at human CRISPR gene editing did not occur in a hospital or University or in a clinical trial by some $100 million funded company. Instead, it happened in small cramped room in San...
View ArticleCultured Meat Will Not Be Realistic Anytime Soon: The Numbers Behind the Hype
Cultured meat, clean meat, synthetic meat, in vitro meat, cellular agriculture, animal free meat, whatever you want to call it, is meat grown in a liquid culture in a bioreactor, a large vat to grow...
View ArticleClik here to view.
Does Heating Antibiotics Destroy Them? No
Whether antibiotics are destroyed by heating is probably inconsequential to most people. However, I see scientists and biohackers talk about it way more than you would expect. Why? In genetic...
View ArticleClik here to view.
Using My Own Blood Serum For Human Tissue Culture
Growing human cells/tissue in culture is one of the foundations of modern medicine. This typical involves taking human cells from an individual or purchasing them obtained from one of many companies...
View ArticleClik here to view.
User Interaction Design for Genetic Engineering: Let's Replace Agar with Bagels
Agar plates are a staple of most labs. They hold moisture well and can have a reasonable distribution of nutrients, chemicals and antibiotics that you may need to grow your organisms. The problem is...
View ArticleClik here to view.
All My Friends Are Dying
My Friend I’m 39 years old and all my friends are dying. The medical system is failing us. Poor and wealthy, those with and without insurance. We suffer at the fate of a system that optimizes for...
View ArticleClik here to view.
Scientists Can't Save Us If There Aren't Many Scientists
Two Normal ScientistsAs a former academic scientist it is hard for me to comprehend how many scientists there actually are. When I was in graduate school I was surrounded by scientists. My friends were...
View ArticleDo-It-Yourself: From Scientific Paper to Covid-19 DNA Vaccine
I'll be honest, when the coronavirus pandemic first started I didn't imagine it would last this long. I am constantly planning and looking to the future and it seemed like some temporary nuisance that...
View ArticleClik here to view.
CRISPR is Dead
Today, the Nobel Prize was awarded for “genome editing” to Emmanuelle Charpentier and Jennifer Doudna. Essentially this was the CRISPR Nobel Prize. If enough of CRISPR has already come so that it is...
View ArticleClik here to view.
I Made a Covid-19 Vaccine in my Kitchen and it Worked - Science Still Sucks
I hate science. It's so elitist. I have an internal dialogue going all the time trying to convince myself that I don't want my work to be called science. What I do is completely different, more sacred,...
View Article
